Clarke's mainstays in John McGinn, Scott McTominay, Billy Gilmour and captain Andy Robertson make up the core of the Tartan Army, who are appearing in their first finals since 1998.
In-form Southampton striker Ross Stewart has earned a recall for the first time since 2022, having caught the eye during the Saints' Premier League promotion push.
Veteran goalkeeper Craig Gordon makes the cut at age 43, while there is also a place for 19-year-old winger Findlay Curtis, who impressed while on loan at Kilmarnock from Rangers.
Scotland's World Cup campaign begins against Haiti on June 14th, before facing Morocco on June 19th and clashing with heavyweights Brazil on June 24th.
Scotland's 2026 World Cup squad:
Goalkeepers: Craig Gordon (Hearts), Angus Gunn (Nottingham Forest), Liam Kelly (Rangers)
Defenders: Grant Hanley (Hibernian), Jack Hendry (Al Etiffaq), Aaron Hickey (Brentford), Dominic Hyam (Wrexham), Scott McKenna (Dinamo Zagreb), Nathan Patterson (Everton), Anthony Ralston (Celtic), Andy Robertson (Liverpool), John Souttar (Rangers), Kieran Tierney (Celtic)
Midfielders: Ryan Christie (Bournemouth), Findlay Curtis (Kilmarnock), Lewis Ferguson (Bologna), Ben Gannon-Doak (Bournemouth), Billy Gilmour (Napoli), John McGinn (Aston Villa), Kenny McLean (Norwich), Scott McTominay (Napoli)
Forwards: Che Adams (Torino), Lyndon Dykes (Charlton), George Hirst (Ipswich), Lawrence Shankland (Hearts), Ross Stewart (Southampton)